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IVF (clinics, countries, procedures, stages)

IVF. Clinics, countries, procedures

IVF is a revolutionary method of assisted reproductive technology that has given great promise to fruitless couples. Currently, it can be considered the most effective method of treating infertility. Sometimes with the help of IVF it is possible to become pregnant even in the most hopeless situations.

On IVF issues we work with different countries and when choosing a country and a doctor, we consider the legislation of each country:

  • Croatia
  • Georgia
  • Germany
  • India
  • Israel
  • Italy
  • Russia (Moscow, Samara)
  • South Korea
  • Spain
  • Switzerland
  • Turkey
  • Ukraine
  • USA

There are donor programs for severe cases: donor egg and donor sperm. We also offer programs of "surrogate motherhood" with full legal support.

IVF Stages

1. Hormonal preparation

Hormonal preparation is a course of intramuscular or subcutaneous injections to stimulate the activity of the ovaries, due to which several eggs are being matured and are subject to further fertilization. This greatly increases the chances of a woman getting pregnant.

2. Ultrasound control

Starting with the day appointed by the doctor, it is necessary to come to the clinic every morning. The woman is doing an ultrasound scan that allows you to monitor the maturation of the follicles and monitor the condition of the uterus and ovaries. Follicles are counted and measured. If the examination shows that the follicles have reached the required size and degree of maturity, in the evening the patient is given an injection of choric gonadotropin (CG), which causes ovulation. The exact time of extraction of eggs occurs on the morning of the second day after the injection - after 34-35 hours.

3. Fertilization

On the day of the "release" of eggs, the partner hands over the sperm for fertilization. Under certain conditions, you can bring sperm with you. Egg collection occurs in the operating room next to the laboratory where fertilization will take place. The patient is under ultrasound control in a gynecological chair. The doctor punctures the follicles through the vagina, and then the eggs are extracted from the follicular fluid obtained and placed in the previously prepared, labeled capsules with the culture medium. Anesthesia for the puncture for most women is not required, but if desired, a puncture can be performed under anesthesia. In the laboratory, the sperm is diluted with a special culture medium and becomes ready for fertilization. a few hours later, a drop of sperm is added to each egg, the mixture is placed overnight in an incubator.

The next day, under a microscope, it is checked whether fertilization has occurred, and if so, how many eggs were fertilized. Fertilized eggs contain two nuclei, in the same nucleus are the chromosomes (hereditary material) of the mother, the other is formed from sperm penetrated into the egg and contains the chromosomes of the father.

4. Embryo transfer to the uterine cavity

Implantation of the embryo into the uterine cavity occurs also in the operating room, in the gynecological chair. This procedure is painless, it can be compared with the usual gynecological examination. A thin, soft catheter with ovules inside it is inserted into the uterus, and then fertilized eggs with a drop of nutrient medium are slowly released from the catheter. After half an hour spent lying down, a woman can go home and continue a normal life. Sex life is allowed from the next day.

Auxiliary hatching

After transfer to the uterus, the fertilized egg continues to divide rapidly, while in the fetal membrane. In order for pregnancy to occur, the embryo must leave it after a few days in order to attach itself to the uterine mucosa.

In accordance with the newest method, the chances of ultimate success can be increased if a tiny hole is made in the fetal shell just before the embryo is implanted. This micromanipulation is performed by means of a specially developed laser device, or mechanically. The method is called "auxiliary hatching" and is used primarily in cases where, during previous attempts, the fertilized egg did not take root in the uterus.

PGD - preimplantation genetic diagnostics

PGD allows genetic testing of the embryo even before transferring it into the uterus. This technique is carried out in the framework of the IVF program and allows the use of only "healthy embryos" that have not identified the desired genetic diseases. First and foremost, PGD is indicated for those couples who are at increased risk of transmitting inherited diseases to the child.

In the laboratory, embryologists grow an embryo for three days. Under the microscope, with the help of micromanipulators, the embryologist selects the material and sends it to genetic research. In the process of studying the genetic content give the conclusion - this embryo is “sick” or not, or what gender it is. After "checking", healthy embryos are transferred to the uterus.

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